A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
A Plastic Biosynthetic Pathway for the Production of Structurally Distinct Microbial Sunscreens (2023)
Arsın, S., Delbaje, E., Jokela, J., Wahlsten, M., Farrar, Z. M., Permi, P., & Fewer, D. (2023). A Plastic Biosynthetic Pathway for the Production of Structurally Distinct Microbial Sunscreens. ACS Chemical Biology, 18(9), 1959-1967. https://doi.org/10.1021/acschembio.3c00112
JYU-tekijät tai -toimittajat
Julkaisun tiedot
Julkaisun kaikki tekijät tai toimittajat: Arsın, Sıla; Delbaje, Endrews; Jokela, Jouni; Wahlsten, Matti; Farrar, Zoë M.; Permi, Perttu; Fewer, David
Lehti tai sarja: ACS Chemical Biology
ISSN: 1554-8929
eISSN: 1554-8937
Julkaisuvuosi: 2023
Ilmestymispäivä: 21.08.2023
Volyymi: 18
Lehden numero: 9
Artikkelin sivunumerot: 1959-1967
Kustantaja: American Chemical Society (ACS)
Julkaisumaa: Yhdysvallat (USA)
Julkaisun kieli: englanti
DOI: https://doi.org/10.1021/acschembio.3c00112
Julkaisun avoin saatavuus: Avoimesti saatavilla
Julkaisukanavan avoin saatavuus: Osittain avoin julkaisukanava
Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/88841
Tiivistelmä
Mycosporine-like amino acids (MAAs) are small, colorless, and water-soluble secondary metabolites. They have high molar extinction coefficients and a unique UV radiation absorption mechanism that make them effective sunscreens. Here we report the discovery of two structurally distinct MAAs from the lichen symbiont strain Nostoc sp. UHCC 0926. We identified these MAAs as aplysiapalythine E (C23H38N2O15) and tricore B (C34H53N4O15) using a combination of high-resolution liquid chromatography–mass spectrometry (HR-LCMS) analysis and nuclear magnetic resonance (NMR) spectroscopy. We obtained a 8.3 Mb complete genome sequence of Nostoc sp. UHCC 0926 to gain insights into the genetic basis for the biosynthesis of these two structural distinct MAAs. We identified MAA biosynthetic genes encoded in three separate locations of the genome. The organization of biosynthetic enzymes in Nostoc sp. UHCC 0926 necessitates a branched biosynthetic pathway to produce two structurally distinct MAAs. We detected the presence of such discontiguous MAA biosynthetic gene clusters in 12% of the publicly available complete cyanobacterial genomes. Bioinformatic analysis of public MAA biosynthetic gene clusters suggests that they are subject to rapid evolutionary processes resulting in highly plastic biosynthetic pathways that are responsible for the chemical diversity in this family of microbial sunscreens.
YSO-asiasanat: bakteerit; biosynteesi; genomiikka; peptidit; proteiinit
Vapaat asiasanat: bacteria; biosynthesis; cluster chemistry; genomics; peptides; proteins
Liittyvät organisaatiot
OKM-raportointi: Kyllä
VIRTA-lähetysvuosi: 2023
JUFO-taso: 2
