A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Effects of unilateral hippocampal surgical procedures needed for calcium imaging on mouse behavior and adult hippocampal neurogenesis (2024)

Lehtonen, S.-M., Puumalainen, V., Nokia, M. S., & Lensu, S. (2024). Effects of unilateral hippocampal surgical procedures needed for calcium imaging on mouse behavior and adult hippocampal neurogenesis. Behavioural Brain Research, 468, Article 115042. https://doi.org/10.1016/j.bbr.2024.115042

JYU-tekijät tai -toimittajat

Lehtonen, Suvi-Maaria
Puumalainen, Veera
Nokia, Miriam
Lensu, Sanna

Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajat: Lehtonen, Suvi-Maaria; Puumalainen, Veera; Nokia, Miriam S.; Lensu, Sanna

Lehti tai sarja: Behavioural Brain Research

ISSN: 0166-4328

eISSN: 1872-7549

Julkaisuvuosi: 2024

Ilmestymispäivä: 07.05.2024

Volyymi: 468

Artikkelinumero: 115042

Kustantaja: Elsevier

Julkaisumaa: Alankomaat

Julkaisun kieli: englanti

DOI: https://doi.org/10.1016/j.bbr.2024.115042

Julkaisun avoin saatavuus: Avoimesti saatavilla

Julkaisukanavan avoin saatavuus: Osittain avoin julkaisukanava

Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/95479

Tiivistelmä

Hippocampus is essential for episodic memory formation, lesion studies demonstrating its role especially in processing spatial and temporal information. Further, adult hippocampal neurogenesis (AHN) in the dentate gyrus (DG) has also been linked to learning. To study hippocampal neuronal activity during events like learning, in vivo calcium imaging has become increasingly popular. It relies on the use of adeno-associated viral (AAV) vectors, which seem to lead to a decrease in AHN when applied on the DG. More notably, imaging requires the implantation of a relatively large lens into the tissue. Here, we examined how injection of an AAV vector and implantation of a 1-mm-diameter lens into the dorsal DG routinely used to image calcium activity impact the behavior of adult male C57BL/6 mice. To this aim, we conducted open-field, object-recognition and object-location tasks at baseline, after AAV vector injection, and after lens implantation. Finally, we determined AHN from hippocampal slices using a doublecortin-antibody. According to our results, the operations needed for in vivo imaging of the dorsal DG did not have adverse effects on behavior, although we noticed a decrease in AHN ipsilaterally to the operations. Thus, our results suggest that in vivo imaging can be safely used to, for example, correlate patterns of calcium activity with learned behavior. One should still keep in mind that the defects on the operated side might be functionally compensated by the (hippocampus in the) contralateral hemisphere.

YSO-asiasanat: hippokampus; kuvantaminen; in vivo -menetelmä; vektorit (epidemiologia); muisti (kognitio); oppiminen; aivotutkimus; koe-eläinmallit

Vapaat asiasanat: adeno-associated viral vector; adult hippocampal neurogenesis; calcium imaging; dentate gyrus; GCaMP

Liittyvät organisaatiot

Hankkeet, joissa julkaisu on tehty

OKM-raportointi: Kyllä

VIRTA-lähetysvuosi: 2024

Alustava JUFO-taso: 1