A1 Journal article (refereed)
Estrogen deficiency reduces maximal running capacity and affects serotonin levels differently in the hippocampus and nucleus accumbens in response to acute exercise (2024)
Lee, E., Nissinen, T. A., Ylä-Outinen, L., Jalkanen, A., Karppinen, J. E., Vieira-Potter, V. J., Lipponen, A., & Karvinen, S. (2024). Estrogen deficiency reduces maximal running capacity and affects serotonin levels differently in the hippocampus and nucleus accumbens in response to acute exercise. Frontiers in Neuroscience, 18, Article 1399229. https://doi.org/10.3389/fnins.2024.1399229
JYU authors or editors
Publication details
All authors or editors: Lee, Earric; Nissinen, Tuuli A.; Ylä-Outinen, Laura; Jalkanen, Aaro; Karppinen, Jari E.; Vieira-Potter, Victoria Jeanne; Lipponen, Arto; Karvinen, Sira
Journal or series: Frontiers in Neuroscience
ISSN: 1662-4548
eISSN: 1662-453X
Publication year: 2024
Publication date: 25/06/2024
Volume: 18
Article number: 1399229
Publisher: Frontiers Media
Publication country: Switzerland
Publication language: English
DOI: https://doi.org/10.3389/fnins.2024.1399229
Publication open access: Openly available
Publication channel open access: Open Access channel
Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/96546
Web address of parallel published publication (pre-print): https://doi.org/10.1101/2024.03.05.582269
Abstract
Methods: We studied the effect of estrogen deficiency induced via ovariectomy (OVX) in female Wistar rats (n = 64). Rats underwent either sham or OVX surgery and were allocated thereafter into four groups matched for body mass and maximal running capacity: sham/control, sham/max, OVX/control, and OVX/max, of which the max groups had maximal running test before euthanasia to induce acute response to exercise. Metabolism, spontaneous activity, and maximal running capacity were measured before (PRE) and after (POST) the surgeries. Three months following the surgery, rats were euthanized, and blood and tissue samples harvested. Proteins were analyzed from gastrocnemius muscle and retroperitoneal adipose tissue via Western blot. Brain neurochemical markers were measured from nucleus accumbens (NA) and hippocampus (HC) using ultra-high performance liquid chromatography.
Results: OVX had lower basal energy expenditure and higher body mass and retroperitoneal adipose tissue mass compared with sham group (p ≤ 0.005). OVX reduced maximal running capacity by 17% (p = 0.005) with no changes in muscle mass or phosphorylated form of regulatory light chain (pRLC) in gastrocnemius muscle. OVX was associated with lower serotonin metabolite 5-hydroxyindoleacetic acid (5-HIAA) level in the NA compared with sham (p = 0.007). In response to acute exercise, OVX was associated with low serotonin level in the HC and high level in the NA (p ≤ 0.024).
Discussion: Our results highlight that OVX reduces maximal running capacity and affects the response of brain neurochemical levels to acute exercise in a brain region-specific manner. These results may offer mechanistic insight into why OVX reduces willingness to exercise.
Keywords: menopause; estrogens; neurochemistry; muscles; adipose tissues; ovaries; running; physical activity
Free keywords: ovariectomy; menopause; neurochemical marker; energy expenditure; skeletal muscle; adipose tissue
Contributing organizations
Related projects
- Estrogenic Regulation of Exercise Response - Role of Extracellular Vesicles and HDL as Signal Carriers
- Karvinen, Sira
- Research Council of Finland
- Exercise, Extracellular vesicles and Senescence (EEVaS)
Molecular mechanisms promoting healthspan- Karvinen, Sira
- Research Council of Finland
Ministry reporting: Yes
Preliminary JUFO rating: 1