A1 Journal article (refereed)
A muramidase from Acremonium alcalophilum hydrolyse peptidoglycan found in the gastrointestinal tract of broiler chickens (2021)

Frederiksen, C. Ø., Cohn, M. T., Skov, L. K., Schmidt, E. G. W., Schnorr, K. M., Buskov, S., Leppänen, M., Maasilta, I., Perez-Calvo, E., Lopez-Ulibarri, R., & Klausen, M. (2021). A muramidase from Acremonium alcalophilum hydrolyse peptidoglycan found in the gastrointestinal tract of broiler chickens. Journal of Industrial Microbiology and Biotechnology, 48(1-2), Article kuab008. https://doi.org/10.1093/jimb/kuab008

JYU authors or editors

Publication details

All authors or editors: Frederiksen, Carsten Østergaard; Cohn, Marianne Thorup; Skov, Lars Kobberøe; Schmidt, Esben Gjerløff Wedebye; Schnorr, Kirk Matthew; Buskov, Steen; Leppänen, Miika; Maasilta, Ilari; Perez-Calvo, Estefania; Lopez-Ulibarri, Rual; et al.

Journal or series: Journal of Industrial Microbiology and Biotechnology

ISSN: 1367-5435

eISSN: 1476-5535

Publication year: 2021

Publication date: 04/02/2021

Volume: 48

Issue number: 1-2

Article number: kuab008

Publisher: Oxford University Press (OUP)

Publication country: United Kingdom

Publication language: English

DOI: https://doi.org/10.1093/jimb/kuab008

Publication open access: Openly available

Publication channel open access: Partially open access channel

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/75308

Abstract

This study evaluates peptidoglycan hydrolysis by a microbial muramidase from the fungus Acremonium alcalophilum in vitro and in the gastrointestinal tract of broiler chickens. Peptidoglycan used for in vitro studies was derived from 5 gram-positive chicken gut isolate type strains. In vitro peptidoglycan hydrolysis was studied by three approaches: a) helium ion microscopy to identify visual phenotypes of hydrolysis b) reducing end assay to quantify solubilization of peptidoglycan fragments and c) mass spectroscopy to estimate relative abundances of soluble substrates and reaction products. Visual effects of peptidoglycan hydrolysis could be observed by helium ion microscopy and the increase in abundance of soluble peptidoglycan due to hydrolysis was quantified by a reducing end assay. Mass spectroscopy confirmed the release of hydrolysis products and identified muropeptides from the five different peptidoglycan sources. Peptidoglycan hydrolysis in chicken crop, jejunum and caecum samples was measured by quantifying the total and soluble muramic acid content. A significant increase in the proportion of the soluble muramic acid was observed in all three segments upon inclusion of the microbial muramidase in the diet.

Keywords: antimicrobial compounds; enzymes; hydrolysis; mould fungi; gastrointestinal microbiota; broilers

Contributing organizations

Ministry reporting: Yes

VIRTA submission year: 2021

JUFO rating: 1